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ecl plus reagent  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc ecl plus reagent
    Ecl Plus Reagent, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ecl+plus+reagent/pmc12966717-110-19-22
    Average 86 stars, based on 1 article reviews
    ecl plus reagent - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    other:

    Article Title: Endotoxemia Engages the RhoA Kinase Pathway to Impair Cardiac Function By Altering Cytoskeleton, Mitochondrial Fission, and Autophagy.
    Article Snippet: Neviere 2 Aims: RhoA/ROCK pathway controls crucial biological processes involved in cardiovascular pathophysiology, such as cytoskeleton dynamics, vascular smooth muscle contraction and inflammation.. In this work, we tested whether Rho kinase inhibition would beneficially impact cardiac cytoskeleton organization, bioenergetics and autophagy in experimental endotoxemia induced by lipopolysaccharides (LPS) in mice.. Results: Fasudil, a potent ROCK inhibitor, prevented LPS-induced cardiac inflammation, oxidative stress, cytoskeleton disarray and mitochondrial injury.

    Incubation:

    Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways
    Article Snippet: Twenty-five micrograms of protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, blocked, and incubated with the following specific antibodies: rabbit monoclonal anti-superoxide dismutase 1 (SOD1) (Cat# PA5-27240, RRID:AB_2544716), rabbit polyclonal anti-IRE1α (Cat# PA1-46027, RRID:AB_2262265), mouse monoclonal anti-CHOP (Cat# MA1-250, RRID:AB_2292611) from Thermo Fisher Scientific; rabbit polyclonal anti-GRP78 (Cat# ab21685, RRID:AB_2119834), mouse monoclonal anti-ATF6 (Cat# ab122897, RRID:AB_10899) from Abcam; rabbit polyclonal anti-eukaryotic initiation factor 2 phospho (p-eIF2α) (Innovative Research Cat# 44-728G, RRID:AB_15000); mouse monoclonal anti-actin (Cell Signaling Technology Cat# 3700, RRID:AB_2242334) or rabbit polyclonal anti-actin (Sigma-Aldrich Cat# A5060, RRID:AB_476738). .. Membranes were incubated with mouse lgG secondary antibodies (Thermo Fisher Scientific Cat# 31430, RRID:AB_228307) and chemiluminescence was detected using ECL Plus reagent (Cell Signalling, Danvers, MA, USA), visualized with a Fusion FX5 system, and quantified by densitometry using Bio1D software ( https://www.vilber.com ,(RRID:SCR_023580). .. Total RNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol.

    Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways.
    Article Snippet: Twenty-five micrograms of protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, blocked, and incubated with the following specific antibodies: rabbit monoclonal anti-superoxide dismutase 1 (SOD1) (Cat# PA5-27240, RRID: AB_2544716), rabbit polyclonal anti-IRE1a (Cat# PA1-46027, RRID:AB_2262265), mouse monoclonal anti-CHOP (Cat# MA1250, RRID:AB_2292611) from Thermo Fisher Scientific; rabbit polyclonal anti-GRP78 (Cat# ab21685, RRID:AB_2119834), mouse monoclonal anti-ATF6 (Cat# ab122897, RRID: AB_10899) from Abcam; rabbit polyclonal anti-eukaryotic initiation factor 2 phospho (p-eIF2a) (Innovative Research Cat# 44-728G, RRID:AB_15000); mouse monoclonal anti-actin (Cell Signaling Technology Cat# 3700, RRID:AB_2242334) or rabbit polyclonal anti-actin (Sigma-Aldrich Cat# A5060, RRID: AB_476738). .. Membranes were incubated with mouse lgG secondary antibodies (Thermo Fisher Scientific Cat# 31430, RRID: AB_228307) and chemiluminescence was detected using ECL Plus reagent (Cell Signalling, Danvers, MA, USA), visualized with a Fusion FX5 system, and quantified by densitometry using Bio1D software (https://www.vilber.com,(RRID: SCR_023580). .. Total RNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol.

    Software:

    Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways
    Article Snippet: Twenty-five micrograms of protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, blocked, and incubated with the following specific antibodies: rabbit monoclonal anti-superoxide dismutase 1 (SOD1) (Cat# PA5-27240, RRID:AB_2544716), rabbit polyclonal anti-IRE1α (Cat# PA1-46027, RRID:AB_2262265), mouse monoclonal anti-CHOP (Cat# MA1-250, RRID:AB_2292611) from Thermo Fisher Scientific; rabbit polyclonal anti-GRP78 (Cat# ab21685, RRID:AB_2119834), mouse monoclonal anti-ATF6 (Cat# ab122897, RRID:AB_10899) from Abcam; rabbit polyclonal anti-eukaryotic initiation factor 2 phospho (p-eIF2α) (Innovative Research Cat# 44-728G, RRID:AB_15000); mouse monoclonal anti-actin (Cell Signaling Technology Cat# 3700, RRID:AB_2242334) or rabbit polyclonal anti-actin (Sigma-Aldrich Cat# A5060, RRID:AB_476738). .. Membranes were incubated with mouse lgG secondary antibodies (Thermo Fisher Scientific Cat# 31430, RRID:AB_228307) and chemiluminescence was detected using ECL Plus reagent (Cell Signalling, Danvers, MA, USA), visualized with a Fusion FX5 system, and quantified by densitometry using Bio1D software ( https://www.vilber.com ,(RRID:SCR_023580). .. Total RNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol.

    Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways.
    Article Snippet: Twenty-five micrograms of protein were separated by SDS-PAGE, transferred to nitrocellulose membranes, blocked, and incubated with the following specific antibodies: rabbit monoclonal anti-superoxide dismutase 1 (SOD1) (Cat# PA5-27240, RRID: AB_2544716), rabbit polyclonal anti-IRE1a (Cat# PA1-46027, RRID:AB_2262265), mouse monoclonal anti-CHOP (Cat# MA1250, RRID:AB_2292611) from Thermo Fisher Scientific; rabbit polyclonal anti-GRP78 (Cat# ab21685, RRID:AB_2119834), mouse monoclonal anti-ATF6 (Cat# ab122897, RRID: AB_10899) from Abcam; rabbit polyclonal anti-eukaryotic initiation factor 2 phospho (p-eIF2a) (Innovative Research Cat# 44-728G, RRID:AB_15000); mouse monoclonal anti-actin (Cell Signaling Technology Cat# 3700, RRID:AB_2242334) or rabbit polyclonal anti-actin (Sigma-Aldrich Cat# A5060, RRID: AB_476738). .. Membranes were incubated with mouse lgG secondary antibodies (Thermo Fisher Scientific Cat# 31430, RRID: AB_228307) and chemiluminescence was detected using ECL Plus reagent (Cell Signalling, Danvers, MA, USA), visualized with a Fusion FX5 system, and quantified by densitometry using Bio1D software (https://www.vilber.com,(RRID: SCR_023580). .. Total RNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol.



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